Composite

Part:BBa_K5477038:Design

Designed by: Kate Malana Escobar   Group: iGEM24_UCopenhagen   (2024-09-28)


CYP3A4-pGAL1/10-POR detox module against a wide array of contaminants


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1483
    Illegal PstI site found at 2486
    Illegal PstI site found at 2683
    Illegal PstI site found at 3067
    Illegal PstI site found at 3507
    Illegal PstI site found at 3567
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2486
    Illegal PstI site found at 2683
    Illegal PstI site found at 3067
    Illegal PstI site found at 3507
    Illegal PstI site found at 3567
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1483
    Illegal PstI site found at 2486
    Illegal PstI site found at 2683
    Illegal PstI site found at 3067
    Illegal PstI site found at 3507
    Illegal PstI site found at 3567
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1483
    Illegal PstI site found at 2486
    Illegal PstI site found at 2683
    Illegal PstI site found at 3067
    Illegal PstI site found at 3507
    Illegal PstI site found at 3567
    Illegal NgoMIV site found at 2889
    Illegal NgoMIV site found at 3008
    Illegal AgeI site found at 1894
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part was cloned using the method of USER-cloning into YCp-H. YCp-H is a centromeric plasmid used in yeast that includes a HIS3 marker, allowing for selection in histidine auxotrophic yeast strains. Like other CEN plasmids, YCp-H contains a CEN sequence, ensuring that the plasmid replicates and segregates similarly to yeast chromosomes. This results in a low copy number (typically one to two copies per cell), providing stable maintenance of the plasmid.


Source

Homo sapiens and S. cerevisiae


References